2s 6 as 6 br 7 s 8 as 8 b s 10 r 11 ar 12 as 12 bs  (Chem Impex International)

Journal: Scientific Reports

Article Title: Antimicrobial effects and mechanisms of hydrogen sulphide against nail pathogens

doi: 10.1038/s41598-025-22062-7

Figure Lengend Snippet: Mechanism of action of H 2 S. ( A ) CLSM images of 12 h pre-incubated T. rubrum ATCC 28,188 exposed to NaHS in RPMI-GM medium (pH 5) for 2 h, at a sulphide concentration that is equivalent to 3.4 µg/mL of H 2 S. The samples were stained with Calcofluor white and DCHF-DA. ( B ) 60 µg/mL of the antioxidants N-acetylcysteine (NAC) and reduced glutathione (GSSH) counteract the effect of H 2 S on T. rubrum . ( C ) Effect of H 2 S on cytochrome c oxidase activity in isolated mitochondria from T. rubrum . Statistical analysis was done by a One-way ANOVA followed by Dunnett’s multiple comparison test (** p < 0.01; *** p < 0.001, n = 3). ( D ) 5-IAF labelling of proteins from T. rubrum conidia, separated by SDS-PAGE. (Left) Coomassie brilliant blue stain of the gel showing molecular weight marker bands (in kDa) and protein extracts from samples that were untreated ( −) or treated with NaHS ( +) for 3 h. (Right) 5-IAF labelled proteins.

Article Snippet: Fig. 5 Mechanism of action of H 2 S. ( A ) CLSM images of 12 h pre-incubated T. rubrum ATCC 28,188 exposed to NaHS in RPMI-GM medium (pH 5) for 2 h, at a sulphide concentration that is equivalent to 3.4 μg/mL of H 2 S. The samples were stained with Calcofluor white and DCHF-DA. ( B ) 60 μg/mL of the antioxidants N-acetylcysteine (NAC) and reduced glutathione (GSSH) counteract the effect of H 2 S on T. rubrum . ( C ) Effect of H 2 S on cytochrome c oxidase activity in isolated mitochondria from T. rubrum .

Techniques: Incubation, Concentration Assay, Staining, Activity Assay, Isolation, Comparison, SDS Page, Molecular Weight, Marker